Difference in dynamics between F-actin and myosin S1 measured by neutron scattering

Matsuo, Tatsuhito; Arata, Toshiaki*; Oda, Toshiro*; Fujiwara, Satoru

no journal, , 

The dynamics of F-actin and myosin S1 were studied by quasi-elastic neutron scattering (QENS). QENS measurements were conducted on DO solution samples of F-actin and S1 at 300 K using AMATERAS in J-PARC. The QENS spectra of proteins were obtained by subtracting those of DO buffer from those of DO samples. In the current analysis, it was found that while the correlation time of atomic motions of S1 was similar to that of other proteins studied so far, the correlation time of F-actin was shorter than S1. Furthermore, F-actin had a population of the atoms undergoing diffusive motions with larger amplitudes than S1. These results suggest that F-actin is more flexible than other proteins including S1.